BioISI Post Graduate Seminars

Auditório da FCiências.ID, Lisboa

Por Tânia Marques e Diana Pimentel (BioSYS PhD Program).


An integrative approach to tissue-specific effects of miRNA regulatory networks - Tânia Marques

MicroRNAs or miRNAs are small noncoding RNAs with a major role in post-transcriptional regulation of gene expression. miRNAs become part of the RISC complex which binds to miRNA responsive elements located predominantly in the 3’UTRs of target mRNAs through base-pairing interactions involving the miRNA seed sequence leading to degradation or translational inhibition of the mRNA molecule. Even though the basic mechanisms for miRNA action have been described, we are still unable to efficiently predict the impact of these molecules on cellular function. miR function has been proposed to be regulated by endogenous competing RNAs, including both coding and noncoding RNA molecules. This suggests that the ability of a given miRNA to act upon a target is dynamically influenced by the transcriptome environment it is in, in addition to the binding affinity it displays to different targets. Thus, when predicting targets for a miRNA, the context in which it is expressed needs to be accounted for. The aim of this work is to comprehensively understand the interaction dynamics between microRNAs and their target transcriptome across different conditions. This will be achieved by dissecting miRNA regulatory networks through correlation analysis between miRNA, mRNA and predicted targets in public datasets for different tissues and conditions.

Transcriptional and metabolic regulation of grape berry metabolism upon powdery mildew infection - Diana Pimentel

Grapevine (Vitis vinifera L.) is very susceptible to several diseases and one of the most problematic is powdery mildew (PM). PM is caused by the obligate biotrophic fungus Erysiphe necator and it affects grapevine yield and berry quality. In this study, naturally infected and control grapes from Carignan variety were collected at green (EL33) and véraison (EL35) stages and metabolic and transcriptomic changes upon PM infection of susceptible grapes were analyzed. Metabolic profiling of control and infected berries was performed by gas chromatography coupled to electron impact ionization/time-of-flight mass spectrometry (GC-EI/TOF-MS) and by solid phase microextraction (SPME) associated with GC-EI-MS. Thirty-six metabolites showed differential content comparing either infected and control samples or green and véraison stages. Among these, twenty were shown to be positive markers of infection including five metabolites that were undetected in control samples. RNAseq analysis was performed to access the expression profile of PM infected and control berries at both stages and 4275 genes were identified as differentially expressed due to infection and/or ripening, and they are mainly associated with response to biotic stress, secondary metabolism (phenylpropanoid and terpene pathways) and hormone metabolism, including salicylic acid and jasmonates. Altogether, these data suggest a metabolic and transcriptomic reprogramming upon infection with PM in particular at green stage.

17h00
BioISI - Instituto de Biossistemas e Ciências Integrativas
Almoços com Ciências

Especialistas de Ciências ULisboa conversam informalmente sobre temas atuais, durante o almoço.

Carlos Alexandre Aleixo Nascimento defende a dissertação "Evaluation of WSN technology for dependable monitoring in water environments".

Por Alan Simcock (United Nations World Ocean Assessment / Former Chairman of OSPAR Commission for the Protection of the Marine Environment of the North East Atlantic).

Gonçalo Alexandre Moleira Domingos defende a dissertação "Smart City Bus: Flexible Transport.AI Front End".

Imagem ilustrativa do evento, sobre um fundo branco

Este seminário destina-se à apresentação de trabalhos em curso sobre as inter-relações entre conhecimento científico, tecnologia e formações imperiais.

Speaker: Marcos Gomes Eleuterio da Luz (Universidade Federal do Paraná, Brasil).

Maria Carolina Serrão Nogueira Nascimento Bulhosa defende a dissertação "The impact of heatwaves on mortality in the Lisbon district - ICARO system revisited".

Orientação: Marília Cristina Antunes, Baltazar Bravo Nunes (INSA).

David Samuel dos Santos Prata defende a dissertação "Caracterização e otimização energética de processos na indústria conserveira - caso de estudo".

Membros do Júri:

Bernardo Faria Gonçalves Pimenta Pedro defende a dissertação "Estudo das atividades biológicas da alga Undaria pinnatifida".

Membros do Júri:

Ana Margarida Santos Antunes defende a dissertação "Development of an ammonia portable low-cost air quality station".

Membros do Júri:

Mariana Dias Simão defende a dissertação "Caracterização da fração glicídica do adesivo produzido pelos pés ambulacrários do ouriço do mar Paracentrotus lividus".

Membros do Júri:

Alda Filipa Queirós Oliveira e Silva defende a dissertação "Single-molecule imaging of transcription dynamics".

Eveline Pereira Almeida defende a dissertação "Utilização de dados geofísicos para identificação de estruturas arqueológicas e manchas de empréstimo de areia em meio marinho: aquisição, processamento e interpretação de dados magnéticos e de sísmica de reflexão

João Samuel Sebastião Silveira Ramos defende a dissertação "Coprocessamento de dados litogeoquímicos e pedogeoquímicos no sector da mina de Argozelo; modelação 2D e 3D e delimitação de domínios anómalos".

Júri:

Daniel Salvador Lourenço defende a dissertação "Desenvolvimento Solução Microsoft para Instituição Financeira".

Leonor Fernanda Venceslau Azeredo Pontes defende a dissertação "Biodiversity informatics - Entomological data processing, analysis and visualization".

Júri das provas:

Teresa Sofia Ferreira Alexandre defende a dissertação "Iteractions of marine protected species with artisanal fisheries in the Parque Natural do Sudoeste Alentejano e Costa Vicentina (PNSACV) and adjacent classified areas (SPAs AND SACs)".

Maria Inês Andrade Elias defende a dissertação "Avaliação da Segurança Alimentar de framboesas irradiadas por feixe de eletrões".

Por Stephan Klaus (M. F. I. Oberwolfach).

Speaker: Pedro Nascimento Alves (Department of Neurosciences and Mental Health, Neurology, Hospital de Santa Maria, CHULN, and Language Research Laboratory, Faculty of Medicine, Universidade de Lisboa).

Por Michael I. Goldstein, PhD (Mike) - US Forest Service, Juneau, Alaska.
Videoconferência

Henrique Martins Espada Niza defende a dissertação "African Marine Invertebrate Data Analysis and Imaging: a Dataset and Digital Platform for Research, Education and Outreach".

Júri das provas:

João David Ferreira de Castro Albuquerque defende a dissertação "Classification methods applied to familial hypercholesterolemia diagnosis in pediatric age".

Imagem de um sinal representativo da intersecção entre a Ciência e a Política, sobre um fundo cinzento claro

O Centro de Filosofia das Ciências da Universidade de Lisboa (CFCUL), em colaboração com o Instituto de Ciências Sociais (ICS-UL), organiza o curso livre “Ciência e Decisão Política em Portugal”, a ter lugar na Fundação Calouste Gulbenkian.

Encontro internacional sobre a conservação da Águia-imperial e outras grandes águias e abutres, visando a divulgação dos resultados obtidos no âmbito do LIFE Imperial e a troca de conhecimentos e de experiências internacionais, relativas às diversas espécies, e que pe

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