BioISI Post Graduate Seminars

Sala 1.3.33A, FCUL, Lisboa

Por Miguel Vitorino (DAEPHYS PhD student, Bio-PhysNano) e Mariana Romão (BioSYS PhD student, FunGP).


Going nano: exploring biological physics through atomic force microscopy

Atomic force microscopes (AFMs) had a critical role in the exploration of the nanoscale over the last decades. In particular, they are fundamental to the latest interdisciplinary effort to study the physics of biological systems at the molecular level. Besides their amazing resolving power, these instruments now serve as true molecular toolboxes, capable of pulling, pushing and manipulating matter as needed. However, these capabilities are still limited when addressing very soft systems, such as mammalian cells, and a quantitative characterization of the forces present in biosystems is still lacking. A solution to these problems may come from a novel AFM-derived technique, called Force Feedback Microscopy (FFM), on which my PhD is centered.

In this talk I will address some of the work performed over the last years, during my PhD. I will cover the technological development of the new instrument and present several examples of research undergone at the AFMRTLab, of BioPhysNano/BioISI. This research covers two main topics: cell mechanics, in the context of gene expression, stress response and disease progression; and molecular interactions and their role in Alzheimer’s disease. Here, FFM seems to offer new avenues for probing these systems and may shed light on the physics that governs life at its fundamental scale.

The S100A9 protein forms new type of string-like polymeric assemblies

Protein aggregation and activation of inflammatory pathways are common features of age-related neurodegenerative disorders. Conversion of misfolded normally soluble proteins into toxic amyloid aggregates is accompanied by upregulation of pro- inflammatory cytokines such as S100 proteins. S100s are small (10-12kDa) dimeric calcium-binding proteins with a dynamic cellular range of intra and extra cellular concentrations (nM to μM) and oligomeric states (2-8mer and higher) which act via regulatory protein:protein interactions, intracellular Ca2+ sensing and signalling processes in inflammation, proliferation and differentiation, cancer and neurodegeneration.
S100 proteins are consistently deregulated in pathophysiological conditions, including Alzheimer’s Disease (AD). In particular, S100A9 is highly abundant in AD brains1 and has been shown to be a relevant AD biomarker2, being upregulated in Tg2576 AD in connection with memory impairment3. S100A9 interacts with amyloid-beta playing role in its aggregation process4.
We gathered evidence that S100A9 forms stable string-like polymeric structures in vitro under physiological conditions. We hypothesize that these polymers, composed by assembly units which can reversibly dissociate, may be a form of protein stabilisation to maximize its extracellular lifetime in the synaptic environment.

[1] Wang, C., et al., Acta Neuropathol, 2014. 127(4): p. 507-22.(1): p. 34-9.
[2] Horvath, I., et al., ACS Chem Neurosci, 2016.
[3] Chang, K.A., H.J. Kim, and Y.H. Suh, Neurodegener Dis, 2012. 10(1-4): p. 27-9.
[4] Zhang, C., et al.. PLoS One, 2012. 7(3): p. e32953

17h00
BioISI - Instituto de Biossistemas e Ciências Integrativas
Frase "O que existe no meu mundo?" sobre um fundo azul e amarelo

Em 2019 celebram-se os 150 anos da Tabela Periódica, pelo que a Organização das Nações Unidas (ONU) proclamou este ano como o Ano Internacional da Tabela Periódica dos Elementos Químicos (IYPT 2019).

Vasco Freire Serra defende a dissertação "Pesquisa e Navegação de Filmes e Músicas com Base em Citações e Letras no AS Music By".

Imagem ilustrativa do Doutoramento em Filosofia da Ciência, Tecnologia, Arte e Sociedade

Provas de Doutoramento - Tiago Bruno Borges Rodrigues Mesquita Carvalho.

Tema da tese e alvo de discussão oral: “A medida e as coisas. Virtudes, responsabilidade e tecnologia”.

Júri:

André Abrantes da Costa defende a dissertação "Computational and validation approaches in proteomics discovery of disease biomarkers".

Júri das provas:

Gonçalo Pimenta Pedro Palmeirim defende a dissertação "The Parental effect in the immune system".

Membros do Júri:

Tânia Filipa Rodrigues Cordeiro defende a dissertação "Identificação de substâncias dopantes em fluidos biológicos de atletas por LC-MS: definição de critérios de identificação e avaliação da incerteza do exame".

Com Dieter Mersch (ZHdK, Suiça), Hanns Holger Rutz (KUG/IEM, Austria) e Miguel Marques Cardoso.

Entrada livre, mediante inscrição prévia.

Rodrigo Daniel Garrilha Santos defende a dissertação "Construction of machine learning models to predict pharmacology properties of molecules".

Júri das provas:

Por Tom Sutherland (Grupo de Física Matemática).

Imagem ilustrativa do evento, acompanhada da palavra "AmeriChristmas" sobre um fundo encarrnado

O concerto conta com a presença da Orquestra Académica da Universidade de Lisboa.

Imagem ilustrativa do programa

A Faculdade de Ciências da Universidade de Lisboa, a Universidade Católica Portuguesa, o Fórum Oceano e a Sociedade de Avaliação Estratégica e Risco lançaram em 2015 o consórcio MARE STARTUP destinado a promover o empreendedorismo e inovação empresarial no Mar,

Miguel Ângelo Teixeira Queirós defende a dissertação "Pneumococcal adaptation: from colonization to disease".

Anabela Barreiro Gomes Vilares Bendada Esteves defende a tese "Genetic study of Toxoplasma gondii strains isolated from humans and animals".

Por Ederson Moreira dos Santos (Universidade de São Paulo - São Carlos - Brazil).

Isabel José Capelo Ferreira Nobre defende a dissertação "Formações e classes de Fitting".

Sara Martins Teixeira defende a dissertação "Diagnosis of Inflammatory Rheumatic Diseases: Preliminary Approach by Metabolomics".

Júri das provas:

Catarina Pires de Almeida Rosa defende a dissertação "Bird talk, the soap opera: vocal and behavioural repertoire of a zoo population of rainbow lorikeets (Trichoglossus moluccanus)".

Júri das provas:

Imagem ilustrativa do evento, acompanhada de várias informações (título, dia, hora, local, orador e entidades participantes)

Por José Díez (Universidad de Barcelona, Espanha).

Cristiana Costa de Almeida defende a dissertação "Previsão de Séries Temporais Financeiras: Uma Abordagem com Long Short-term Memory Deep Neural Networks".

Diogo Domingues Ferreira defende a dissertação "Avaliação e otimização da incerteza de constantes cinéticas de fotodegradação determinadas pelo Método de Monte Carlo".

Membros do Júri:

Miguel Simões Nunes defende a dissertação "Understanding the differentiation process of western Mediterranean butterflies: the case studies of Lycaena and Melanargia".

Júri das provas:

Por Rogério Henriques (Presidente da Multicare).

Por Joana Portugal Pereira (Universidade Federal do Rio de Janeiro, Imperial College London e IPCC).

Páginas