BioISI Post Graduate Seminars

Sala 1.3.33A, FCUL, Lisboa

Por Miguel Vitorino (DAEPHYS PhD student, Bio-PhysNano) e Mariana Romão (BioSYS PhD student, FunGP).


Going nano: exploring biological physics through atomic force microscopy

Atomic force microscopes (AFMs) had a critical role in the exploration of the nanoscale over the last decades. In particular, they are fundamental to the latest interdisciplinary effort to study the physics of biological systems at the molecular level. Besides their amazing resolving power, these instruments now serve as true molecular toolboxes, capable of pulling, pushing and manipulating matter as needed. However, these capabilities are still limited when addressing very soft systems, such as mammalian cells, and a quantitative characterization of the forces present in biosystems is still lacking. A solution to these problems may come from a novel AFM-derived technique, called Force Feedback Microscopy (FFM), on which my PhD is centered.

In this talk I will address some of the work performed over the last years, during my PhD. I will cover the technological development of the new instrument and present several examples of research undergone at the AFMRTLab, of BioPhysNano/BioISI. This research covers two main topics: cell mechanics, in the context of gene expression, stress response and disease progression; and molecular interactions and their role in Alzheimer’s disease. Here, FFM seems to offer new avenues for probing these systems and may shed light on the physics that governs life at its fundamental scale.

The S100A9 protein forms new type of string-like polymeric assemblies

Protein aggregation and activation of inflammatory pathways are common features of age-related neurodegenerative disorders. Conversion of misfolded normally soluble proteins into toxic amyloid aggregates is accompanied by upregulation of pro- inflammatory cytokines such as S100 proteins. S100s are small (10-12kDa) dimeric calcium-binding proteins with a dynamic cellular range of intra and extra cellular concentrations (nM to μM) and oligomeric states (2-8mer and higher) which act via regulatory protein:protein interactions, intracellular Ca2+ sensing and signalling processes in inflammation, proliferation and differentiation, cancer and neurodegeneration.
S100 proteins are consistently deregulated in pathophysiological conditions, including Alzheimer’s Disease (AD). In particular, S100A9 is highly abundant in AD brains1 and has been shown to be a relevant AD biomarker2, being upregulated in Tg2576 AD in connection with memory impairment3. S100A9 interacts with amyloid-beta playing role in its aggregation process4.
We gathered evidence that S100A9 forms stable string-like polymeric structures in vitro under physiological conditions. We hypothesize that these polymers, composed by assembly units which can reversibly dissociate, may be a form of protein stabilisation to maximize its extracellular lifetime in the synaptic environment.

[1] Wang, C., et al., Acta Neuropathol, 2014. 127(4): p. 507-22.(1): p. 34-9.
[2] Horvath, I., et al., ACS Chem Neurosci, 2016.
[3] Chang, K.A., H.J. Kim, and Y.H. Suh, Neurodegener Dis, 2012. 10(1-4): p. 27-9.
[4] Zhang, C., et al.. PLoS One, 2012. 7(3): p. e32953

17h00
BioISI - Instituto de Biossistemas e Ciências Integrativas

Seminário do Laboratório de Instrumentação e Física Experimental de Partículas, por Jonathan Hollar (LIP).

This colloquium seeks to debate ideas from the arts, humanities and sciences on the process of empathy.

Neuroscience and Neuromodeling Seminar, por Athanasios Vourvopoulos (LaSEEB, ISR, IST-ULisboa).

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No dia 6 de outubro, a ULisboa dá as boas-vindas aos seus estudantes na Cidade Universitária!

Geodiversidade

Palestra no âmbito das comemorações do Dia Internacional da Geodiversidade, por Carlos Marques da Silva (DG Ciências ULisboa).

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Celebrando o início de um novo Ano Académico, esta sessão solene é um importante marco na vida académica da ULisboa e junta toda a sua Comunidade.

Imagem ilustrativa do curso, acompanhada de diversas informações

Um curso dirigido ao público geral e estudantes de arte ou ciência (a partir dos 16 anos), com interesse em desenho de observação, ilustração, e biologia, com ou sem experiência em desenho.

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Talk @LASIGE, por Bruno Loff (Faculdade de Ciências, Universidade do Porto).

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The main goal of this annual conference is to expose students, engineers, scientists and the overall academic and scientific communities to the greatest recent advances in the fields of Technology, Health and Biomedical Engineering taking place in some of the most prestigious research institutions.

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Participe até 12 de outubro / Submit you application until 12 October.

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Ciências ULisboa estará presente, de 13 a 16 de outubro, no Festival Internacional de Ciência - FIC.A

Seminário Permanente de Filosofia das Ciências, por James Ladyman (University of Bristol).

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A ciência rege-se por diferentes escalas de tempo. Diferentes áreas do conhecimento estudam processos a diferentes escalas: dos nanossegundos aos milhões de anos. Quais são os Andamentos da Ciência?

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Um evento repleto de atividades científicas, culturais e educativas, com jogos, demonstrações, palestras, entre muitas outras atividades. 

Research at the Faculty of Sciences

Join us on 26 October 2022 for the 4th edition of Ciências Research Day.