BioISI Post Graduate Seminars

Sala 1.3.33A, FCUL, Lisboa

Por Miguel Vitorino (DAEPHYS PhD student, Bio-PhysNano) e Mariana Romão (BioSYS PhD student, FunGP).


Going nano: exploring biological physics through atomic force microscopy

Atomic force microscopes (AFMs) had a critical role in the exploration of the nanoscale over the last decades. In particular, they are fundamental to the latest interdisciplinary effort to study the physics of biological systems at the molecular level. Besides their amazing resolving power, these instruments now serve as true molecular toolboxes, capable of pulling, pushing and manipulating matter as needed. However, these capabilities are still limited when addressing very soft systems, such as mammalian cells, and a quantitative characterization of the forces present in biosystems is still lacking. A solution to these problems may come from a novel AFM-derived technique, called Force Feedback Microscopy (FFM), on which my PhD is centered.

In this talk I will address some of the work performed over the last years, during my PhD. I will cover the technological development of the new instrument and present several examples of research undergone at the AFMRTLab, of BioPhysNano/BioISI. This research covers two main topics: cell mechanics, in the context of gene expression, stress response and disease progression; and molecular interactions and their role in Alzheimer’s disease. Here, FFM seems to offer new avenues for probing these systems and may shed light on the physics that governs life at its fundamental scale.

The S100A9 protein forms new type of string-like polymeric assemblies

Protein aggregation and activation of inflammatory pathways are common features of age-related neurodegenerative disorders. Conversion of misfolded normally soluble proteins into toxic amyloid aggregates is accompanied by upregulation of pro- inflammatory cytokines such as S100 proteins. S100s are small (10-12kDa) dimeric calcium-binding proteins with a dynamic cellular range of intra and extra cellular concentrations (nM to μM) and oligomeric states (2-8mer and higher) which act via regulatory protein:protein interactions, intracellular Ca2+ sensing and signalling processes in inflammation, proliferation and differentiation, cancer and neurodegeneration.
S100 proteins are consistently deregulated in pathophysiological conditions, including Alzheimer’s Disease (AD). In particular, S100A9 is highly abundant in AD brains1 and has been shown to be a relevant AD biomarker2, being upregulated in Tg2576 AD in connection with memory impairment3. S100A9 interacts with amyloid-beta playing role in its aggregation process4.
We gathered evidence that S100A9 forms stable string-like polymeric structures in vitro under physiological conditions. We hypothesize that these polymers, composed by assembly units which can reversibly dissociate, may be a form of protein stabilisation to maximize its extracellular lifetime in the synaptic environment.

[1] Wang, C., et al., Acta Neuropathol, 2014. 127(4): p. 507-22.(1): p. 34-9.
[2] Horvath, I., et al., ACS Chem Neurosci, 2016.
[3] Chang, K.A., H.J. Kim, and Y.H. Suh, Neurodegener Dis, 2012. 10(1-4): p. 27-9.
[4] Zhang, C., et al.. PLoS One, 2012. 7(3): p. e32953

17h00
BioISI - Instituto de Biossistemas e Ciências Integrativas
Feixes luminosos

Envio de propostas até 20 de junho.

Seminário do Laboratório de Instrumentação e Física Experimental de Partículas, por Rui Santos (ISEL / CFTC-UL / LIP).

Título/data/local do evento e pormenor de ilustração do livro

Um livro composto por um ensaio da historiadora e professora Ana Simões e uma banda desenhada da artista Ana Matilde Sousa, concebido como complemento à exposição E3 - Einstein, Eddington, e o Eclipse.

Seminário "Ásia-Europa, 1500-1800", por Ricardo Roque (ICS-ULisboa), Matheus Serva Pereira (ICS-ULisboa) e José Miguel Moura Ferreira (IHC-UNL).

Título/data do evento e fotografia do orador

TWIN2PIPSA Expert Seminar, por Danny Hatters (The University of Melbourne).

Mini-Course, por Mahir Bilen Can (Tulane Univ, USA).

An opportunity to get acquainted with some of the most promising contemporary topics in the exciting interdisciplinary area of scientific culture: the interactions of mathematics and music.

Título/data/local/orador do evento e representação da mente humana

Minicurso por Heliton Tavares (Universidade Federal do Pará, Brasil).

Por Omiros Papaspiliopoulos, (Department of Decision Sciences, Bocconi University). 

Título/data/local do evento e imagem representativa de pessoa a trabalhar num mundo tecnológico

As Jornadas Científicas 2024 da Universidade de Lisboa são dedicadas ao tema “Impacto Atual e Futuro da Inteligência Artificial no Trabalho”.

Lisbon AI Seminar, por Klaus Gärtner (CFCUL/RG2).

Lisbon Webinar in Analysis and Differential Equations, por Adán Corcho (Universidad de Córdoba).

Pessoa ajoelhada no hall de edifício pejado de luzes

Ação de formação para docentes e investigadores de Ciências.

Seminário Helena Avelar de Astronomia e Astrologia Antiga, por Stephen Johnston (History of Science Museum, University of Oxford).

Seminário do Laboratório de Instrumentação e Física Experimental de Partículas, por Paschal Coyle (Centre de Physique de Particules de Marseille).

Título/data/local do evento, sobre a Tabela Periódica

This year's program will cover two plenary sessions hosted by Susete Pinteus and Hugo Miranda, complemented by oral presentations, flash talks, and poster communications. Finally, a round table discussion will take place at the end of our meeting.

Logótipo Moodle

Ação de formação para docentes e investigadores de Ciências.

Vai realizar-se em Lisboa, nos dias 28 e 29 de junho de 2024, o 37.º Encontro do Seminário Nacional de História da Matemática.

Logótipo do prémio

As candidaturas à 11.ª edição decorrem até 28 de junho.

Título/data do evento e fotografia da oradora

TWIN2PIPSA Expert Seminar, por Isabelle Landrieu (Lille University, Inserm, Institute Pasteur de Lille Research Department U1167).

Logótipo do Verão na ULisboa, sobre um fundo amarelo

Uma oportunidade única de conheceres e experimentares o ritmo e o espírito da vida académica!

Reasoning Seminar, por Joseph Berkovitz (Institute for the History and Philosophy of Science and Technology and Philosophy Department University of Toronto).

Título/data/local do evento e representação do cérebro humano

O maior evento anual na área da ciência e da tecnologia em Portugal.

Workshop no âmbito do projeto EVORISE, por Kevin Mitchell (Smurfit Institute of Genetics and Institute of Neuroscience, Trinity College Dublin) e Luís Pessoa (Department of Psychology, University of Maryland).

The topics of the conference include (but are not limited to) classical and quantum integrable systems, complex geometry of moduli spaces, automorphic forms and their applications to number theory.

Páginas