BioISI Post Graduate Seminars

Sala 1.3.33A, FCUL, Lisboa

Por Miguel Vitorino (DAEPHYS PhD student, Bio-PhysNano) e Mariana Romão (BioSYS PhD student, FunGP).


Going nano: exploring biological physics through atomic force microscopy

Atomic force microscopes (AFMs) had a critical role in the exploration of the nanoscale over the last decades. In particular, they are fundamental to the latest interdisciplinary effort to study the physics of biological systems at the molecular level. Besides their amazing resolving power, these instruments now serve as true molecular toolboxes, capable of pulling, pushing and manipulating matter as needed. However, these capabilities are still limited when addressing very soft systems, such as mammalian cells, and a quantitative characterization of the forces present in biosystems is still lacking. A solution to these problems may come from a novel AFM-derived technique, called Force Feedback Microscopy (FFM), on which my PhD is centered.

In this talk I will address some of the work performed over the last years, during my PhD. I will cover the technological development of the new instrument and present several examples of research undergone at the AFMRTLab, of BioPhysNano/BioISI. This research covers two main topics: cell mechanics, in the context of gene expression, stress response and disease progression; and molecular interactions and their role in Alzheimer’s disease. Here, FFM seems to offer new avenues for probing these systems and may shed light on the physics that governs life at its fundamental scale.

The S100A9 protein forms new type of string-like polymeric assemblies

Protein aggregation and activation of inflammatory pathways are common features of age-related neurodegenerative disorders. Conversion of misfolded normally soluble proteins into toxic amyloid aggregates is accompanied by upregulation of pro- inflammatory cytokines such as S100 proteins. S100s are small (10-12kDa) dimeric calcium-binding proteins with a dynamic cellular range of intra and extra cellular concentrations (nM to μM) and oligomeric states (2-8mer and higher) which act via regulatory protein:protein interactions, intracellular Ca2+ sensing and signalling processes in inflammation, proliferation and differentiation, cancer and neurodegeneration.
S100 proteins are consistently deregulated in pathophysiological conditions, including Alzheimer’s Disease (AD). In particular, S100A9 is highly abundant in AD brains1 and has been shown to be a relevant AD biomarker2, being upregulated in Tg2576 AD in connection with memory impairment3. S100A9 interacts with amyloid-beta playing role in its aggregation process4.
We gathered evidence that S100A9 forms stable string-like polymeric structures in vitro under physiological conditions. We hypothesize that these polymers, composed by assembly units which can reversibly dissociate, may be a form of protein stabilisation to maximize its extracellular lifetime in the synaptic environment.

[1] Wang, C., et al., Acta Neuropathol, 2014. 127(4): p. 507-22.(1): p. 34-9.
[2] Horvath, I., et al., ACS Chem Neurosci, 2016.
[3] Chang, K.A., H.J. Kim, and Y.H. Suh, Neurodegener Dis, 2012. 10(1-4): p. 27-9.
[4] Zhang, C., et al.. PLoS One, 2012. 7(3): p. e32953

17h00
BioISI - Instituto de Biossistemas e Ciências Integrativas

O curso pretende fornecer as ferramentas necessárias para que o investigador na área da saúde consiga desenvolver autonomia para o desenvolvimento de trabalhos de investigação científica utilizando a metodologia estatística apropriada.

Fotografia de palestra

Ana Subtil Simões (Ciências ULisboa) e Joana Sousa (Faculdade de Medicina da ULisboa) serão as oradoras. As inscrições são efetuadas através deste link

Speaker: Alexandra B. Moura​ (ISEG/UL - Universidade de Lisboa, Department of Mathematics; REM - Research in Economics and Mathematics, CEMAPRE).​

De onde vêm todos os compostos que te permitem estar a ver esta mensagem ou notícia no teu smartphone ou computador? Será possível montá-lo sem toda uma cadeia de impactes sociais e económicos?

O principal objetivo desta iniciativa centra-se em atrair fotógrafos profissionais e amadores a submeter trabalhos de excelência produzidos em Portugal e nos países africanos de língua oficial portuguesa (PALOP) e fomentar práticas de observação, conhecimento e registo através

De onde vêm todos os compostos que te permitem estar a ver esta mensagem ou notícia no teu smartphone ou computador? Será possível montá-lo sem toda uma cadeia de impactes sociais e económicos?

O cE3c - Centro de Ecologia, Evolução e Alterações Ambientais está mais uma vez a organizar o Seminário cE3c I, que irá decorrer no 1.º semestre do ano letivo 2020/2021 na Faculdade de Ciências.

Ciências ULisboa associa-se ao maior evento mundial sobre inovação para a sustentabilidade - “The Planetiers World Gathering” - que terá lugar no Altice Arena entre 21 e 23 de outubro de 2020, sob os auspícios da

Fotografia de caminhantes numa montanha

Suspensão motivada pela aplicação do plano de contingência da Faculdade de Ciências da Universidade de Lisboa, na sequência das orientações da Direção-Geral de Saúde e das recomendações por parte da Reitoria da Universidade de Lisboa, relativamente ao novo Coronavírus (COVID

De onde vêm todos os compostos que te permitem estar a ver esta mensagem ou notícia no teu smartphone ou computador? Será possível montá-lo sem toda uma cadeia de impactes sociais e económicos?

Fotografia de ilha no Oceano Pacífico

A Direção-Geral de Política do Mar, na qualidade de operador do Programa Crescimento Azul do Mecanismo Financeiro do Espaço Económico Europeu 2014/2021 (EEA Grants), lançou recentemente o

Leitura e escrita são a base fundacional de todas as literacias e a condição primeira para o juízo compreensivo e crítico, o enriquecimento cultural, a formação estética e a memória individual e coletiva das sociedades.

Objectives: Evolutionary theory provides a framework for understanding all living systems.

Overview: Currently, climate changes or alterations are known to be reflected on the stable isotope ratios of Hydrogen, Nitrogen, Carbon, Oxygen and Sulphur present in atmospheric gas forms, fresh or ocean water, as well as in plants and animals and organi

Objectives: To introduce participants to the details of communicating science to non-specialized audiences, including, but not exclusive to, public and private stakeholders, students and teachers, and media professionals.

De onde vêm todos os compostos que te permitem estar a ver esta mensagem ou notícia no teu smartphone ou computador? Será possível montá-lo sem toda uma cadeia de impactes sociais e económicos?

Objectives: Natural history museums are privileged spaces for seminal research on different subjects of biological sciences such as biodiversity, evolution, ecology, biogeography and taxonomy.

De onde vêm todos os compostos que te permitem estar a ver esta mensagem ou notícia no teu smartphone ou computador? Será possível montá-lo sem toda uma cadeia de impactes sociais e económicos?

Objectives: Provide students with basic workflows, platforms and tools to increase reproducibility at all scientific levels.

Objectives: Under the general framework of Global Change Ecology, the goal of this course is to provide the participants with the most recent and practical knowledge on the use of Functional Diversity.

Objectives: The objective of this course is to introduce participants to the details of communication and writing scientific publications. The main emphasis is on the most common form, the “primary scientific paper”, but other forms will be covered.

Objectives: Lack of fertile land to feed the exponentially growing population, insufficient water availability and quality, changes in the flow of nutrients through the bio-geo-cycles (especially N and P) and climate and land use changes are impacting ecosystem

Objectives: Symbiosis is a key strategy for life on Earth.

Objectives: The goal of this course is to provide to the participants with current and practical knowledge on urban ecology, including ecological and social aspects. It aims at providing an integrated approach on urban socio-ecological systems.

Objectives: Citizen science can be part of the larger process of engaging people in new forms of interaction in scientific research, challenging scientists and citizens whilst enlarging scientific knowledge and providing learning opportunities for all part

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