Featuring expert speakers from Instruct Centres across Europe, Instruct-ERIC Webinar Series: Structure Meets Function highlights some of the latest developments in structural biology, demonstrating how integrative methods are enabling scientists to decipher the mechanisms that underpin health and disease.
Vídeo do evento
Carlos Cordeiro (Ciências ULisboa) foi um dos oradores do webinar, com a apresentação "Breaking the limits of native MS with MRMS":
Native MS is a fundamental methodology for the structural characterisation of macromolecules, providing information on its mass, stability, and dynamics, from minute amounts of sample. Among its applications are the determination of macromolecular complexes stoichiometry, small molecule binding, and protein and complex assemblies, from small proteins to large viral particles. Native MS is often preformed on highly tuned, modified mass spectrometers that preserve structure and interactions in the gas phase. However, resolutions fall short of the achievable potential of any of these instruments mainly due to gas-phase desolvation and intrinsic resolving power of the mass spectrometers. Magnetic Resonance Mass Spectrometry (MRMS) provides the highest resolving power of any other mass spectrometer, allowing for the extensive manipulation and diversity of fragmentation methods for structural interrogation of gas-phase macromolecular complexes. Here we show the potential and applications of MRMS in the field of structural biology.
