Overview: Currently, climate changes or alterations are known to be reflected on the stable isotope ratios of Hydrogen, Nitrogen, Carbon, Oxygen and Sulphur present in atmospheric gas forms, fresh or ocean water, as well as in plants and animals and organic matter in the soil. Samples from those matrices can provide a record for such changes across a given length of time and / or space. Also, ecological and physiological processes often reflect on stable isotope ratios, again setting a record in plant, animal or other living tissues. Many such processes will establish typical “isotopic signatures” which can allow for a geographical discrimination of the origin (or growth) of a given living tissue; at the same time, the time scales involved in establishing those isotopic markers will depend upon metabolic turnover time of a given isotope, residence time of a given organism or cycling time in the ecosystem. For those, and other, reasons, stable isotopes ratios are widely used in diverse ecological areas of research, integrating multidisciplinary approaches together with biochemistry, molecular biology, physiology geochemistry and climate sciences.

Objectives: Introduction to stable isotopes ratios as tools to understand global, ecosystem and community level bio/geo cycles;Light isotopes: H, N, C, O and S isotopes, geo cycles and ecological significance;Sampling and analytical methodologies in stable isotope analysis.

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