Por Vincent Carré (Université de Lorraine).

Plants are fascinating organisms at many levels. First, by their nature, they are a very interesting source of organic compounds that can be directly used or after conversion. Second, in their environment, plants live in a complex equilibrium with other abiotic systems, including microorganisms such as bacteria and fungi. This equilibrium is dynamic and involves multiple interactions that can induce the synthesis of compounds with interesting properties for medicine, agronomy, and environmental protection. To discover and understand this molecular wealth, a comprehensive analysis of the raw plant or its conversion products can be relevant. This may involve a systemic approach to a particular stage in the development of a plant in its complex environment. This is a particular line research at the LCP-A2MC laboratory of the University of Lorraine. We are developing "omics" mass spectrometry methods to decipher the composition and dynamics of very complex systems such as plants. Our approach involves the use of very high-resolution mass spectrometry (HRMS), namely Fourier transform ion cyclotron resonance mass spectrometry. In the last 10 years, we have implemented the spatial dimension by using MALDI HRMS to probe and image this molecular diversity in grapevine leaves. In this presentation, we will focus on the recent methodological works that we perform in the laboratory in these fields. In particular, we will discuss the analytical workflow and how it can be improved to obtain more relevant information on different samples, considering the molecular diversity of the samples and the complexity of the MS data.

Note: The talk will be held by Dr. Vicent Carré but Dr. Jasmine Hertzog, co-author of this work, will also be available for discussion and Q&As.